Lab tests


  • Colostrum IgG (immunoglobulin G)

Colostrum is a fluid produced by all mammals in the first few days after giving birth, before breast milk is produced. Raising healthy calves is a vital part of dairy farming. Survival and wellbeing of newborn calves is strongly dependent on the quality of colostrum fed at birth since colostrum is the vehicle for antibody transfer from the dam to the newborn. Inadequate immunoglobulin (antibodies) in colostrum leads to failure of passive transfer of immunity and consequently to higher mortality and morbidity rates. Farmers either test and use only colostrum of good quality or rely on quality ensured commercial products.  Since colostrum quality is defined by the IgG levels there is a need for a reliable and efficient assay to measure this vital colostrum component .


CPS's solution, named STIGA (Split TreA Immunoglobulin G Assay), consists of TreAsure Assay reagents that detect bovine immunoglobulins and give a quantitative colorimetric reaction. The ready-to-use reagents are freeze‐dried in microtiter plates. Just add sample. The test is fast (<1h) and requires no further manipulation (no rinsing etc.).

This new developed technology has many benefits over the currently used test (Radial Immune Diffusion or RID) in that it is more consistent, less time consuming, without the need for high level trained technicians and does not rely on the use of reagents produced in live animals (e.g. rabbits).


                                        Order information for Colostrum TreAsure Assay


  • Serum IgG

Testing of total immunoglobulin level in blood or serum of calves can demonstrate the success or failure of transfer of maternal antibodies. This test can be used to evaluate colostrum management practices on dairy and beef farms. 


                                        Order information for Serum TreAsure Assay

                                            

  • Vaccination efficacy test

Vaccine-specific antibodies can be detected in blood or colostrum of vaccinated animals to assess the protection of the vaccinated animal or their offspring. The immune protection from colostrum comes predominantly from the specific antibodies raised by the dam against infectious agents and vaccines. Currently, the level of protection afforded by colostrum (and colostrum products) is usually assumed based upon the total amount of IgG in the product, however this protection could be more accurately predicted with assays that measure the levels of the pathogen-specific antibodies. 


                                        Order information for E. coli (K99) Antibodies TreAsure Assay 

                                           

  • Infectious diseases

The TreAsure Assays can replace antibody ELISAs for many production animal infectious diseases. TreAsure Assays have the advantage that they are easy to use and require little processing time.


                                        Request info on pathogen-specific antibody TreAsre Assays

                                   

  • Biomarkers

The health status of animals can be evaluated based on specific biomarkers. TreAsure Assays can be developed for virtually any protein biomarker in clinical samples. 

  

A first protein biomarker TreAsure Assay was developed to quantify lactoferrin levels (LF) in milk. Lactoferrin has been demonstrated to be a good indicator for clinical and subclinical mastitis. In persistent bacterial intramammary infections, lactoferrin levels increase strongly in milk. A strong correlation also exists between pathogen numbers in milk and the level of lactoferrin. Importantly, lactoferrin concentrations in milk are significantly positively correlated with Somatic Cell Counts, with increases in LF concentration preceding the SCC increases. 

                          


                                        Request info on Lactoferrin TreAsure Assays

                                           

                                        Request info on Custom Biomarker TreAsure Assay



  • Hypocalcemia and hypercalcemia

A very sensitive calcium concentration TreAsure Assay was developed for free calcium quantification in blood, milk and water. This assay can be used to detect low calcium levels (Milk Fever) or high calcium levels in clinical samples from humans and animals.


                                        Request info on calcium TreAsre Assays

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* Currently for research or in-house testing only.