Tre-Assure Assay Technology
OVERVIEW
- Creative Protein Solutions (CPS) has created biosensors for infectious diseases and inflammation biomarkers, using the patent-pending TreAsure Assay technology, developed at the University of Calgary.
- This new approach relies on split enzyme complementation and produces glucose as the signalling molecule, which can be easily measured using existing or custom made glucometers.
- The enzyme trehalase (TreA), split into two non-functional fragments, is fused to specific sensing elements. For detecting antibodies, the enzyme fragments are fused with specific antigens. For detecting biomarkers of chronic disease, other sensing elements are incorporated.
- Binding of the sensing element to the antibodies or biomarkers leads to complementation and activation of TreA, which will catalyse the hydrolysis of the substrate trehalose, resulting in the creation of glucose molecules.
- This glucose level is proportional with antibodies/biomarkers in the sample, giving a precise reading to your cellphone through our smartphone App. Frequent testing allows the user to monitor the level of the target molecule with ease.
Lab-based TreAsure Assays convert glucose into a colorimetric signal.
Point-of-Care TreAsure Assays can be integrated with existing tech or CPS's novel glucometer technology.
Publications: Our technology has been peer-reviewed:
- Split trehalase as a versatile reporter for a wide range of biological analytes. Drikic M, De Buck J. Biotechnology and Bioengineering. 2018, 115:1128–1136.
- Determining the IgG concentrations in bovine colostrum and calf sera with a novel enzymatic assay. M. Drikic, C. Windeyer, S. Olsen, Y. Fu, L. Doepel and J. De Buck. Journal of Animal Science and Biotechnology. 2018, 9:69.
